目的 比较黄精不同炮制品挥发性特征气味物质的差异,为建立黄精不同炮制品的鉴别方法和进一步建立黄精不同炮制品质量控制的新方法提供思路。方法 采用气相色谱-离子迁移谱法(gas chromatography-ion mobility spectrometry, GC-IMS)对黄精不同炮制品的挥发性物质进行检测并比较成分变化,结合主成分分析(principal component analysis, PCA)和“最近邻”指纹分析(nearest neighbor fingerprint analysis,NNFA)分析其挥发性物质的差异。结果 GC-IMS指纹图谱显示黄精不同炮制品的挥发性物质存在差异,明确定性的挥发性物质有62种单体及部分物质的二聚体;1-丙醇、2-庚酮、2-正戊基呋喃、1-己醇、乙酸甲酯、1-戊烯-3-醇、3-甲基丁醇等可作为黄精的特征挥发性物质;5-甲基糠醛、2-乙酰基呋喃、当归内酯、辛醛、糠醛和2-甲基四氢呋喃-3-酮等可作为蒸黄精的特征挥发性物质;1,8-桉树脑等可作为酒黄精的特征挥发性物质;PCA和NNFA结果显示,各组样品分离度良好。结论 GC-IMS技术结合聚类分析方法证明黄精不同炮制品所含挥发性物质存在差异,研究结果可为黄精不同炮制品的辨识提供一定的思路。
Abstract
OBJECTIVE To compare the difference of volatile specific odor components of Polygonati Rhizoma in different processed products, so as to provide research ideas for establishing the identification methods of different processed products of Polygonati Rhizoma and further establish a new method for quality control of different processed products of Polygonati Rhizoma. METHODS Gas chromatography-ion mobility spectrometry(GC-IMS) was used to detect and compare the volatile components of Polygonati Rhizoma in different processed products, and compare the composition changes. Principal component analysis(PCA)and nearest neighbor fingerprint analysis(NNFA) were used to analyze the differences in volatile components. RESULTS GC-IMS fingerprints showed that the volatile components of Polygonati Rhizoma in different processed products were different.It was identified that the volatile components included 62 monomers and dimers of some substances.1-Propanol,heptan-2-one, 2-pentylfuran,1-hexanol, methyl acetate, 1-penten-3-ol and 3-methyl-1-butanol could be used as the characteristic volatile substances of Polygonati Rhizoma.5-Methylfurfural,2-acetylfuran, 5-methyl-2(3H)-furanone, octanal, furfural and 2-methyl-dihydro-(2H)-furan-3-one could be used as characteristic flavor substances ofsteamed Polygonati Rhizoma. 1,8-Cineole could be used as characteristic flavor substances of wine steamed Polygonati Rhizoma. The results of PCA and NNFA showed that the resolution of each group of samples was good. CONCLUSION The GC-IMS technology combined with cluster analysis proves that the volatile components of Polygonati Rhizoma in different processed products are different, which provides certain ideas for the identification of different processed products of Polygonati Rhizoma.
关键词
黄精 /
1-丙醇 /
1 /
8-桉树脑 /
气相色谱-离子迁移谱 /
蒸黄精 /
酒蒸黄精
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Key words
Polygonati Rhizoma /
1-propanol /
1,8-cineole /
gas chromatography-ion mobility spectrometry /
steamed Polygonati Rhizoma /
wine steamed Polygonati Rhizoma
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中图分类号：
R282
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参考文献
[1] GONG Q F. Processing of Chinese Materia Medica(中药炮制学) [M]. 4th ed. Beijing: China Press of Traditional Chinese Medicine Co., Ltd., 2016: 313-314.
[2] QU S H, CHENG X L, PAN Y N, et al. The history of origin processing and processing methods of Polygonati Rhizoma[J]. J Shenyang Pharm Univ(沈阳药科大学学报), 2020, 37(4): 379-384.
[3] LIU X Q, YI H, YAO L, et al. Advances in plants of polygonatum and discussion of its development prospects[J]. Chin Pharm J(中国药学杂志), 2017, 52(7):530-534.
[4] XU J X, LIU L, YANG S X, et al. Chemical constituents from aerial part of molygonatumcyrtonema[J]. Chin Tradit Herb Drugs(中草药), 2016, 47(20):3569-3572.
[5] SUN J, SONG Y J, WANG C L, et al. Quality standard for pieces of polygonatirhizoma processed with steamed[J]. Res Pract Chin Med(现代中药研究与实践), 2016, 30(2): 57-60.
[6] SONG Y J, GUO T, MA C D, et al. Optimization of high pressure steaming technology for processed rhizomapolygonati by response surface methodology[J]. Mod Tradit Chin Med Mater Med World Sci Technol(世界科学技术-中医药现代化), 2018, 20(7): 1261-1267.
[7] DING Y C. Study on the effect analysis and processing progress of Chinese traditional medicine of Polygonati Rhizoma[J]. Guangming J Chin Med(光明中医), 2021, 36(15): 2647-2649.
[8] QIN Y W, ZHANG L P, ZHAO Q, et al.Research progress on Polygonatum cyrtonema processed by nine times steaming and nine times shining[J]. Chin Tradit Herb Drugs(中草药), 2020, 51(21): 5631-5637.
[9] LI J J,WANG H J,MENG D Y, et al. Research on the history of the processing of huangjing[J]. Hubei Agric Sci(湖北农业科学), 2020, 59(Suppl 1): 171-173,176.
[10] FENG X, YIN L H, JIN S H, et al. Ion mobility spectrometry and its applications in pharmaceutical research and development[J]. Chin J Pharm Anal(药物分析杂志), 2013, 33(7): 1109-1114.
[11] CONNIE M G, JOHN C R,JOHN F K, et al.Detection of undeclared erectiledysfunction drugs and analogues in dietary supplements by ion mobility spectrometry [J]. J Pharm Biomed Anal, 2009, 49(3): 601-606.
[12] LI K W, ZHANG C, XU J Y, et al. Research advance in gas detection of volatile organic compounds released in rice quality deterioration process[J]. Compr Rev Food Sci Food Saf, 2021, 20:5802-5828.
[13] CHEN Y J, YU J N, JING G X, et al. Application of gas chromatography-ion mobility spectrometry in agriculture[J]. Chin J Anal Lab(分析试验室), 2020, 39(12): 1480-1488.
[14] YUAN Z Y, QU H Y, XIE M Z, et al. Direct authentication of three Chinese materia Medica species of the Lilii Bulbus family in terms of volatile components by headspace-gas chromatography-ion mobility spectrometry[J]. Anal Methods, 2019, 11(4): 530-536.
[15] LV W, LIN T, REN Z Y, et al. Rapid discrimination of Citrus reticulata ‘Chachi’ by headspace-gas chromatography-ion mobility spectrometry fingerprints combined with principal component analysis[J]. Food Res Int, 2020, 131: 108985.
[16] FENG J W,GUO X H,ZHANG Y, et al. GC-IMS analysis of volatile components in RhododendriMollis Flos at different flowering stages[J]. Chin J Exp Tradit Med Form(中国实验方剂学杂志), 2021, 27(10): 85-92.
[17] MA Y,WANG Y Y,GUAN M R, et al. Changes of the color and odor of the flowers from Lonicera japonica in different development[J]. Chin Tradit Pat Med(中成药), 2019, 41(6): 1349-1353.
[18] JIAO H R, ZHANG M M, ZHAO H Q, et al. Effect of different hot air drying methods on chemical constituents of trichosanthis fructus[J]. Chin J Exp Tradit Med Form(中国实验方剂学杂志), 2021, 27(23): 137-144.
[19] HE J, YE L H,LI J H, et al. Analysis of VOCs in zhemaidong before and after processing by headspace-gas chromatography-ion migration spectrum[J]. Anal Instrum(分析仪器), 2021,(1): 103-108.
[20] LIN X M, YUE L D, ZHANG Z L, et al. Using GC-IMS to compare difference of specific odor components of Angelicae Sinensis Radix by different yellow wine processing methods[J]. Chin Tradit Herb Drugs(中草药), 2020, 51(17): 4464-4472.
[21] LI M Y, SHEN T C, LIU C F, et al. Research on the quality difference of wolfberry from different production areas[J]. Food Ferment Ind(食品与发酵工业), 2021, 47(24):56-63.
[22] LIN S Z,DU F M,LIN L J, et al. Monitoring variation of volatile components in semen armeniacaeamarum under accelerated damage conditions by GC-IMS Technology[J]. J Guangzhou Univ Tradit Chin Med(广州中医药大学学报), 2019, 36(8): 1247-1251.
[23] YANG B Y,LUO Y,QI H Y, et al. Analysis of volatile organic compounds of raw and honey baked Farfarae Flos based on headspace-gas-chromatography ion-mobility spectrometry[J].Chin Tradit Herb Drugs(中草药), 2022, 53(6): 1854-1861.
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脚注
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基金
国家自然科学基金项目资助(82104395);陕西省科技厅青年项目资助(2021JQ-736);陕西省科技厅项目资助(20215F-389)
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